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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide <t>CRISPR</t> screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.
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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide <t>CRISPR</t> screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.
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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide <t>CRISPR</t> screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.
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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide <t>CRISPR</t> screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.
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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide <t>CRISPR</t> screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.
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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide <t>CRISPR</t> screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.
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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide <t>CRISPR</t> screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.
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Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide CRISPR screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.

Journal: Cell Reports Medicine

Article Title: CDK4/6 inhibition overcomes venetoclax resistance mechanisms with enhanced combination activity in acute myeloid leukemia

doi: 10.1016/j.xcrm.2025.102526

Figure Lengend Snippet: Genetic targeting of protein synthesis pathways sensitizes AML cells to venetoclax (A) Volcano plots of genome-wide CRISPR screen. All 480 genes uniquely sensitizing to ven with no evidence of an effect by combination treatment (non-significant) are shown as black dots. (B–D) Reactome pathways from differential expression analysis (DEA) of the 480 gene set show enrichment in pathways related to cell cycle (B), metabolism of RNA (C), and metabolism of proteins (D). The dot size indicates the number of evaluated genes in the Reactome pathway.

Article Snippet: Virus for sgRNA YUSA library was produced by transfecting HEK 293T/17 cells with Calcium chloride, HEPES buffer saline, VSVG, psPAX2 and human CRISPR library plasmid DNA (Addgene #67989).

Techniques: Genome Wide, CRISPR, Quantitative Proteomics